Part:BBa_K4375006:Design

Anti-CEA Nanobody (C43)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 244
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 244
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 244
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 244
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

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Source

We obtained the sequence from: Reprogramming Synthetic Cells for Targeted Cancer Therapy Boon Lim, Yutong Yin, Hua Ye, Zhanfeng Cui, Antonis Papachristodoulou, and Wei E. Huang ACS Synthetic Biology 2022 11 (3), 1349-1360 DOI: 10.1021/acssynbio.1c00631